Genotyping of tick-borne pathogens and determination of human attacking tick species in Novosibirsk and its suburbs

The south of Western Siberia traditionally belongs to the regions with unfavorable epidemiological situation for tick-transmitted infections. In Novosibirsk and its suburbs, approximately 20 thousand people annually seek medical care after tick bites. purpose this work was determine human-attacking species composition in territory suburbs by identifying nucleotide sequence mitochondrial gene cytochrome oxidase COI fragment during 2018 spring-summer season, determination infections individual ticks viral bacterial tick-borne infections, followed genotyping identified pathogens PCR methods, determining sequences phylogenetic analysis. It is established that five attack humans: Ixodes persulcatus, pavlovskyi, Dermacentor reticulatus, marginatus, nuttali metropolis. majority attacks on humans are associated I. pavlovskyi (43.6%) D. reticulatus (41.2%) ticks. genetic material encephalitis virus detected 3.6%, borrelia 13.8% Rickettsia 23.1% collected from humans. Genotyping (TBEV) showed presence TBEV Siberian Far Eastern genotype 83% 17% cases, respectively. Kemerovo could not be detected. Genetic Borrelia spp. has been all attacking local human population. bulk isolates were genotyped as garinii (86%) 13% afzelii species. miyamotoi found one tick. Ticks genus have infected raoultii, largely tarasevichiae, persulcatus helvetica a single case. Thus, population attacked ixodid dominated following found: genotype, B. garinii, afzelii, miyamotoi, R. raoulti, tarasevichiae